Mutation of lysine 48 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. Ub K48R can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K48 linked.
Product Information
Stock:
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW:
9.3 kDa
Purity:
> 95% by PAGE
Use & Storage
Use:
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Storage:
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.
