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Product Description
Mutation of lysine 48 to  cystine  renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. Ub K48C can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K48 linked.