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GST Ubiquitin Mutant K63R
Cat. # UBM‐GK63R
    Mutation of lysine 63 to arginine renders ubiquitin (Ub) unable to form poly‐Ub chains via lysine 63 linkages with
other Ub molecules. UBM GK63R with an N‐terminal GST‐tag (glutathione S‐transferase). GST‐Ub allows for glutathione
affinity purification of ubiquitinated molecules and allows for immuno‐ detection of conjugates using GST
antibodies. UBM GK63R can form an E1‐catalyzed active thioester at the C‐terminus allowing the molecule to be
transferred to the lysines of substrate proteins. Ideal for the reduction in poly‐Ub chain length/conjugation rates and
determining if poly‐Ub chains are K63 linked